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calm1234  (Addgene inc)


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    Structured Review

    Addgene inc calm1234
    A Representative MIP image of the apical surface area of a MCC expressing calmodulin-GFP. Calmodulin displays colocalization with the basal bodies and the apical actin network. B Schematic depicting the localization of calmodulin in a MCC in relation to the basal bodies and the apical actin network. C Representative images of the skin epithelium from stage 24 control and calmodulin inhibitor-treated embryos. MCC fail to fully integrate into the superficial skin epithelium when calmodulin activity is blocked, as evident by their small apical surface area. D Quantification of the apical surface area of MCCs from control ( n = 100 MCCs) and calmodulin inhibitor-treated embryos ( n = 100 MCCs), 5 different embryos. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. E Representative images of the skin neuroepithelium from embryos expressing wild-type calmodulin or Ca 2+ binding deficient calmodulin mutant <t>(CALM1234).</t> Expression of CALM1234 results in defective MCC apical emergence (green arrows) while expression of WT calmodulin does not affect MCC apical emergence (white arrows). F Quantification of MCC apical emergence upon expression of WT and Ca 2+ binding deficient mutant calmodulin. Two-sided χ 2 test; ** * * p < 0.0001. G Quantification of the apical surface area of MCCs expressing WT and Ca 2+ binding deficient mutant calmodulin. N = 80 control MCCs, 46 MCCs expressing WT calmodulin and 20 MCCs expressing mutant calmodulin from 4 embryos injected with WT calmodulin DNA and 4 embryos injected with mutant CALM1234 DNA. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. Scale bars: A : 2 μm, D , F : 20 μm. Source data are provided as a Source data file.
    Calm1234, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calm1234/CaM1234(D20A%2FD56AD93A%2FD129A)%2FpIRES2-eGFP+(Plasmid+%23111518)/pmc12274442-426-17-18
    Average 93 stars, based on 3 article reviews
    calm1234 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Calcium transients regulate the apical emergence of basally located progenitors during Xenopus skin development"

    Article Title: Calcium transients regulate the apical emergence of basally located progenitors during Xenopus skin development

    Journal: Nature Communications

    doi: 10.1038/s41467-025-61610-7

    A Representative MIP image of the apical surface area of a MCC expressing calmodulin-GFP. Calmodulin displays colocalization with the basal bodies and the apical actin network. B Schematic depicting the localization of calmodulin in a MCC in relation to the basal bodies and the apical actin network. C Representative images of the skin epithelium from stage 24 control and calmodulin inhibitor-treated embryos. MCC fail to fully integrate into the superficial skin epithelium when calmodulin activity is blocked, as evident by their small apical surface area. D Quantification of the apical surface area of MCCs from control ( n = 100 MCCs) and calmodulin inhibitor-treated embryos ( n = 100 MCCs), 5 different embryos. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. E Representative images of the skin neuroepithelium from embryos expressing wild-type calmodulin or Ca 2+ binding deficient calmodulin mutant (CALM1234). Expression of CALM1234 results in defective MCC apical emergence (green arrows) while expression of WT calmodulin does not affect MCC apical emergence (white arrows). F Quantification of MCC apical emergence upon expression of WT and Ca 2+ binding deficient mutant calmodulin. Two-sided χ 2 test; ** * * p < 0.0001. G Quantification of the apical surface area of MCCs expressing WT and Ca 2+ binding deficient mutant calmodulin. N = 80 control MCCs, 46 MCCs expressing WT calmodulin and 20 MCCs expressing mutant calmodulin from 4 embryos injected with WT calmodulin DNA and 4 embryos injected with mutant CALM1234 DNA. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. Scale bars: A : 2 μm, D , F : 20 μm. Source data are provided as a Source data file.
    Figure Legend Snippet: A Representative MIP image of the apical surface area of a MCC expressing calmodulin-GFP. Calmodulin displays colocalization with the basal bodies and the apical actin network. B Schematic depicting the localization of calmodulin in a MCC in relation to the basal bodies and the apical actin network. C Representative images of the skin epithelium from stage 24 control and calmodulin inhibitor-treated embryos. MCC fail to fully integrate into the superficial skin epithelium when calmodulin activity is blocked, as evident by their small apical surface area. D Quantification of the apical surface area of MCCs from control ( n = 100 MCCs) and calmodulin inhibitor-treated embryos ( n = 100 MCCs), 5 different embryos. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. E Representative images of the skin neuroepithelium from embryos expressing wild-type calmodulin or Ca 2+ binding deficient calmodulin mutant (CALM1234). Expression of CALM1234 results in defective MCC apical emergence (green arrows) while expression of WT calmodulin does not affect MCC apical emergence (white arrows). F Quantification of MCC apical emergence upon expression of WT and Ca 2+ binding deficient mutant calmodulin. Two-sided χ 2 test; ** * * p < 0.0001. G Quantification of the apical surface area of MCCs expressing WT and Ca 2+ binding deficient mutant calmodulin. N = 80 control MCCs, 46 MCCs expressing WT calmodulin and 20 MCCs expressing mutant calmodulin from 4 embryos injected with WT calmodulin DNA and 4 embryos injected with mutant CALM1234 DNA. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. Scale bars: A : 2 μm, D , F : 20 μm. Source data are provided as a Source data file.

    Techniques Used: Expressing, Control, Activity Assay, Binding Assay, Mutagenesis, Injection

    Related Articles

    Microinjection:

    Article Title: Calcium transients regulate the apical emergence of basally located progenitors during Xenopus skin development
    Article Snippet: .. For microinjection of DNA constructs, atub:UtrGFP, atub:GECO-RED, PACR (Addgene #55774), atub:PACR, CALM-GFP (Addgene #47602), CALMWT (Addgene #111499), CALM1234 (Addgene #111518). .. Par3GFP, GFP-PKC-γ-C1a (Addgene #21205), PH-PLCD1 (Addgene #21179), Cameleon (Addgene #51961) we injected 80 pg of DNA per blastomere.

    Article Title: Calcium transients regulate epithelial integration of multiciliated cells during Xenopus skin development
    Article Snippet: .. For microinjection of DNA constructs, atub:UtrGFP, atub:GECO-RED, PACR (Addgene #55774 ) , atub:PACR, CALM-GFP (Addgene #47602), CALMWT (Addgene #111499), CALM1234 (Addgene #111518). .. Par3GFP, GFP-PKC-γ-C1a(Addgene #21205), PH-PLCD1 (Addgene #21179) we injected 80pg of DNA per blastomere.

    Construct:

    Article Title: Calcium transients regulate the apical emergence of basally located progenitors during Xenopus skin development
    Article Snippet: .. For microinjection of DNA constructs, atub:UtrGFP, atub:GECO-RED, PACR (Addgene #55774), atub:PACR, CALM-GFP (Addgene #47602), CALMWT (Addgene #111499), CALM1234 (Addgene #111518). .. Par3GFP, GFP-PKC-γ-C1a (Addgene #21205), PH-PLCD1 (Addgene #21179), Cameleon (Addgene #51961) we injected 80 pg of DNA per blastomere.

    Article Title: Calcium transients regulate epithelial integration of multiciliated cells during Xenopus skin development
    Article Snippet: .. For microinjection of DNA constructs, atub:UtrGFP, atub:GECO-RED, PACR (Addgene #55774 ) , atub:PACR, CALM-GFP (Addgene #47602), CALMWT (Addgene #111499), CALM1234 (Addgene #111518). .. Par3GFP, GFP-PKC-γ-C1a(Addgene #21205), PH-PLCD1 (Addgene #21179) we injected 80pg of DNA per blastomere.



    Similar Products

    calm1234  (Addgene inc)
    93
    Addgene inc calm1234
    A Representative MIP image of the apical surface area of a MCC expressing calmodulin-GFP. Calmodulin displays colocalization with the basal bodies and the apical actin network. B Schematic depicting the localization of calmodulin in a MCC in relation to the basal bodies and the apical actin network. C Representative images of the skin epithelium from stage 24 control and calmodulin inhibitor-treated embryos. MCC fail to fully integrate into the superficial skin epithelium when calmodulin activity is blocked, as evident by their small apical surface area. D Quantification of the apical surface area of MCCs from control ( n = 100 MCCs) and calmodulin inhibitor-treated embryos ( n = 100 MCCs), 5 different embryos. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. E Representative images of the skin neuroepithelium from embryos expressing wild-type calmodulin or Ca 2+ binding deficient calmodulin mutant <t>(CALM1234).</t> Expression of CALM1234 results in defective MCC apical emergence (green arrows) while expression of WT calmodulin does not affect MCC apical emergence (white arrows). F Quantification of MCC apical emergence upon expression of WT and Ca 2+ binding deficient mutant calmodulin. Two-sided χ 2 test; ** * * p < 0.0001. G Quantification of the apical surface area of MCCs expressing WT and Ca 2+ binding deficient mutant calmodulin. N = 80 control MCCs, 46 MCCs expressing WT calmodulin and 20 MCCs expressing mutant calmodulin from 4 embryos injected with WT calmodulin DNA and 4 embryos injected with mutant CALM1234 DNA. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. Scale bars: A : 2 μm, D , F : 20 μm. Source data are provided as a Source data file.
    Calm1234, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calm1234/CaM1234(D20A%2FD56AD93A%2FD129A)%2FpIRES2-eGFP+(Plasmid+%23111518)/pmc12274442-426-17-18
    Average 93 stars, based on 1 article reviews
    calm1234 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    A Representative MIP image of the apical surface area of a MCC expressing calmodulin-GFP. Calmodulin displays colocalization with the basal bodies and the apical actin network. B Schematic depicting the localization of calmodulin in a MCC in relation to the basal bodies and the apical actin network. C Representative images of the skin epithelium from stage 24 control and calmodulin inhibitor-treated embryos. MCC fail to fully integrate into the superficial skin epithelium when calmodulin activity is blocked, as evident by their small apical surface area. D Quantification of the apical surface area of MCCs from control ( n = 100 MCCs) and calmodulin inhibitor-treated embryos ( n = 100 MCCs), 5 different embryos. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. E Representative images of the skin neuroepithelium from embryos expressing wild-type calmodulin or Ca 2+ binding deficient calmodulin mutant (CALM1234). Expression of CALM1234 results in defective MCC apical emergence (green arrows) while expression of WT calmodulin does not affect MCC apical emergence (white arrows). F Quantification of MCC apical emergence upon expression of WT and Ca 2+ binding deficient mutant calmodulin. Two-sided χ 2 test; ** * * p < 0.0001. G Quantification of the apical surface area of MCCs expressing WT and Ca 2+ binding deficient mutant calmodulin. N = 80 control MCCs, 46 MCCs expressing WT calmodulin and 20 MCCs expressing mutant calmodulin from 4 embryos injected with WT calmodulin DNA and 4 embryos injected with mutant CALM1234 DNA. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. Scale bars: A : 2 μm, D , F : 20 μm. Source data are provided as a Source data file.

    Journal: Nature Communications

    Article Title: Calcium transients regulate the apical emergence of basally located progenitors during Xenopus skin development

    doi: 10.1038/s41467-025-61610-7

    Figure Lengend Snippet: A Representative MIP image of the apical surface area of a MCC expressing calmodulin-GFP. Calmodulin displays colocalization with the basal bodies and the apical actin network. B Schematic depicting the localization of calmodulin in a MCC in relation to the basal bodies and the apical actin network. C Representative images of the skin epithelium from stage 24 control and calmodulin inhibitor-treated embryos. MCC fail to fully integrate into the superficial skin epithelium when calmodulin activity is blocked, as evident by their small apical surface area. D Quantification of the apical surface area of MCCs from control ( n = 100 MCCs) and calmodulin inhibitor-treated embryos ( n = 100 MCCs), 5 different embryos. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. E Representative images of the skin neuroepithelium from embryos expressing wild-type calmodulin or Ca 2+ binding deficient calmodulin mutant (CALM1234). Expression of CALM1234 results in defective MCC apical emergence (green arrows) while expression of WT calmodulin does not affect MCC apical emergence (white arrows). F Quantification of MCC apical emergence upon expression of WT and Ca 2+ binding deficient mutant calmodulin. Two-sided χ 2 test; ** * * p < 0.0001. G Quantification of the apical surface area of MCCs expressing WT and Ca 2+ binding deficient mutant calmodulin. N = 80 control MCCs, 46 MCCs expressing WT calmodulin and 20 MCCs expressing mutant calmodulin from 4 embryos injected with WT calmodulin DNA and 4 embryos injected with mutant CALM1234 DNA. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. Scale bars: A : 2 μm, D , F : 20 μm. Source data are provided as a Source data file.

    Article Snippet: For microinjection of DNA constructs, atub:UtrGFP, atub:GECO-RED, PACR (Addgene #55774), atub:PACR, CALM-GFP (Addgene #47602), CALMWT (Addgene #111499), CALM1234 (Addgene #111518).

    Techniques: Expressing, Control, Activity Assay, Binding Assay, Mutagenesis, Injection